In an interview with Moviehole, Chuck actor Ryan McPartlin confirmed to the site that he recently auditioned for the role of Steve Rogers in The First Avenger: Captain America.

"The one thing I can't stand are these actors that are twittering about auditioning for Captain America. It's really tacky.
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With that said, it is in the casting process and like many other actors, I read for it, so we'll wait and see what happens. Regardless, I'm pretty psyched to be Captain Awesome [on Chuck] for the time being."

Brian B. at Movieweb
Brian B.